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Appreciably raised troponin stages, significantly those that rise and drop over a series of hours, are a powerful sign of a heart injuries.
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The captured troponin is then reacted with a second antibody and, in some assays, a 3rd antibody coupled to an indicator molecule.[36] The assays vary from one another by the categories of antibody applied, with the epitopes to which they bind, and by the kind of indicator molecule that's employed.[forty six]
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Results can be confounded if there is very poor preanalytical handling of troponin specimens, such as topanwin login incomplete sample mixing at enough time of specimen collection, inadequate sample centrifugation and separation of red cells from serum or plasma, presence of fibrin due to incomplete serum separation, and so forth.